FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3694634
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCAGAGATCTCGTAT71600.19379456801404416TruSeq Adapter, Index 1 (97% over 36bp)
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT38020.1029059982666754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC13500.047.70215647
CGTATGC13600.046.5793746
TATGCCG14050.046.0839148
TCGTATG14500.042.7227545
ATGCCGT15100.042.4158449
ACCAGAG19250.034.18070635
TGCCGTC19600.033.21320750
CTCGTAT17950.032.75659644
CACAACC21350.030.8203431
GCCGTCT20750.030.36045851
CAACCAG23300.028.69005633
CTGCTTG22150.028.6010759
AGTCACA23600.028.17895928
TGCTTGA23250.027.54898360
CAGTCAC23900.027.38627227
ACAACCA24150.027.24696732
TCTCGTA21950.026.62783643
ACGTCTG26650.026.5282215
CCGTCTT25100.025.93541352
CCAGTCA25600.025.84145226
Produced by FastQC (version 0.11.5)