FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3009093
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTATCTCGTAT67600.22465241187294643TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9350.052.4041448
GTATGCC9650.050.41315547
CGTATGC9800.049.64152546
CACGTTA12800.045.3894136
TCGTATG10750.041.3477345
ATGCCGT11600.038.92072349
ACGTTAT14850.038.88784437
CTCGTAT13000.034.7298444
ACTCCAC17250.034.49469432
CCACGTT17050.034.48587835
TGCCGTC13200.032.3470850
CTCCACG18750.031.5458233
GTTATCT17100.030.0864139
GTCACTC20250.029.38388329
CGTTATC19100.029.31809838
CAGTCAC20300.028.96714627
GCCGTCT14100.027.55199651
CACTCCA22200.026.96097831
TCACTCC22650.026.42488930
CTGCTTG16100.026.30495559
Produced by FastQC (version 0.11.5)