FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691663
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTATCTCGTAT104230.38723272564210304TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14000.057.2480746
GTATGCC14050.057.04434247
TATGCCG14650.054.23025548
TCGTATG15650.050.988745
ATGCCGT15950.049.37137249
CGAACTA17200.047.61467436
CTCGTAT17950.044.2603544
AGTCACG18950.043.4062628
TGCCGTC18250.043.34100750
ACTATCT18350.041.1975239
CACGACG20600.040.43853831
ACGAACT20600.039.92584235
CTGCTTG19450.039.22965659
GCCGTCT19750.039.1632451
AACTATC20250.038.02340738
TCACGAC21900.037.87895630
CAGTCAC21650.037.83133727
GTCACGA22150.037.60944729
CTATCTC20100.037.4365540
GACGAAC22450.036.94753334
Produced by FastQC (version 0.11.5)