FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989414
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGTAT55760.18652485068980074TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA8400.049.99862735
CGTATGC8950.048.8813446
TATGCCG9300.047.41725548
GTATGCC9450.047.40610547
TCGTATG9950.043.9686445
CCGCTTA10800.039.5359534
ATGCCGT11800.037.07463549
CTTAAAT11650.036.05111737
CACCCGC11800.035.89064831
GCTTAAA12100.034.13129436
ACCCGCT12500.033.32019832
CTCGTAT13000.032.57602744
GTCACCC13600.032.42720829
TGCCGTC14750.030.84609650
TTAAATC13750.030.54461738
TCACCCG14200.029.82462130
GCCGTCT15400.029.08962251
CCCGCTT14850.028.75342233
CTGCTTG16500.026.09106459
CCGTCTT18400.025.10758652
Produced by FastQC (version 0.11.5)