FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4561607
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGTCATATCTCGTAT103280.2264114378989685TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC15600.052.72252747
CGTATGC16050.051.24432446
TATGCCG16550.049.483648
ATGCCGT17500.046.59735549
TCGTATG18750.043.67848245
CGTCATA20150.041.1652736
GTCATAT22000.037.54538337
AGTCACG22450.037.4184628
TGCCGTC23400.034.99801650
CTGCTTG23950.034.48807559
CTCGTAT23650.033.740944
CATATCT25150.032.28516439
GTCACGA26600.031.97537629
TCACGAC27150.031.19870630
ACGACGT27500.031.0565332
GCCGTCT26150.031.04986451
CACGACG27700.030.8329731
CAGTCAC28000.030.25292827
ACGTCTG30350.028.36773315
TGCTTGA29450.028.28486460
Produced by FastQC (version 0.11.5)