FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3116299
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGGAATATCTCGTAT86200.2766101712319646TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10850.054.83810446
GTATGCC11000.053.45395347
TATGCCG11150.053.04704348
TCGTATG12850.046.30299445
ATGCCGT12700.045.4704849
TGCCGTC15100.037.31627750
ACGGAGG19400.036.44523632
AGTCACG20050.034.7405628
GTCACGG20600.033.9823929
CTCGTAT17850.032.7447444
GCCGTCT16800.031.45697651
CACGGAG22900.031.02785131
CAGTCAC23900.029.58363727
CTGCTTG17050.028.53524259
AGGAATA24550.028.0847836
CCAGTCA25700.028.05641726
ACTCCAG26400.027.84328323
TCTCGTA21850.027.71136743
TGCTTGA18000.027.6125860
TCACGGA26050.027.27592330
Produced by FastQC (version 0.11.5)