FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8544547
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGTAT181330.21221721877122332TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC28550.048.91265547
TATGCCG28200.048.8988948
CGTATGC29050.047.82982346
TCGTATG32550.042.68683245
TAGCATC31250.041.77444538
GGATAGC34650.041.6158335
ATGCCGT33650.040.5631349
ATAGCAT35450.039.0970137
AGGATAG38750.037.573934
CAGGATA40700.035.9456633
CTCGTAT41100.032.6145544
TCACAGG45750.032.13258430
TGCCGTC43950.031.53466450
GATAGCA45300.031.52292836
CACAGGA47000.031.20407131
ACAGGAT47750.030.49386632
GTCACAG50050.029.44187729
CTGCTTG46250.028.98389259
GCCGTCT46950.028.84876851
AGTCACA53950.027.5730628
Produced by FastQC (version 0.11.5)