FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6527596
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCTCGTAT111180.17032304082544325TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC18700.051.46977646
GTATGCC19200.050.31170747
TATGCCG20500.047.11976248
TCGTATG22550.042.83746345
ATGCCGT23700.040.60993249
CCTCATG28400.034.0135535
CTCGTAT27850.033.679944
TGCCGTC29650.032.34251450
GCCGTCT33500.028.83448251
CTGCTTG34350.028.1222659
CACTCCT37300.026.27505931
GATCTCG34400.025.9445641
GTCACTC39100.025.87034629
TGCTTGA38450.025.66969160
CAGTCAC38550.025.60467127
TCTCGTA35700.025.48979443
AGTCACT39300.025.02639428
CTCCTCA42200.023.63706833
CATGATC37250.023.5829838
ACTCCTC41450.023.55957832
Produced by FastQC (version 0.11.5)