FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4802317
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCTCGTAT70280.14634602422122486TruSeq Adapter, Index 15 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC12600.045.83223747
TATGCCG13500.042.77586448
CGTATGC13350.042.47089446
ATGCCGT16300.035.8572949
TCGTATG14900.035.23405545
TGCCGTC20400.028.47911650
CTCGTAT18450.026.55762944
GTCACAT27600.024.4760929
GCCGTCT23200.023.68428451
CAGTCAC27500.023.67437727
ATCTGAC27200.023.5477234
CTGACCA25900.023.5134436
AGTCACA28700.023.29406528
TCTGACC27250.022.99075535
CTGCTTG23600.022.69084459
TGACCAT26950.022.33759337
GACCATC26100.020.65063338
CACATCT33250.020.52817531
ACTCCAG34050.020.35426723
CCAGTCA32200.020.3281426
Produced by FastQC (version 0.11.5)