FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408225
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCTCGTAT50240.20861838075761194TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC7550.051.91845347
ACCTTAG8450.048.8788632
TATGCCG8250.047.93747348
CCTTAGG8750.047.60006733
CGTATGC8250.047.0890246
ATGCCGT9050.043.31318349
CTTAGGA10000.041.3000634
TCGTATG9500.041.261545
TAGGACA10400.040.38467436
TTAGGAC10800.038.88894735
CTCGTAT10350.035.50582544
GGACATC11750.032.16892638
AGTCACC13450.031.22745928
AGGACAT12900.030.93027937
TGCCGTC13000.029.88341950
CTGCTTG13600.028.30945459
CAGTCAC15450.027.86527627
GCCGTCT14500.027.033451
ACGTCTG16350.026.75789315
TCTCGTA13950.025.84125943
Produced by FastQC (version 0.11.5)