FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7980893
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATCTCGTAT168520.21115431568873308TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC28600.051.5196647
CGTATGC28800.050.6757846
TATGCCG29400.049.75969348
TCGTATG32150.045.28655645
ATGCCGT34250.042.71343649
CTCGTAT38850.036.2152944
TGCCGTC42950.034.387350
CACCATG43950.033.9270231
CATGAGC43850.033.76217734
CCATGAG44450.033.54265633
GCCGTCT46800.031.40886351
AGTCACC48050.031.1041728
CTGCTTG45550.030.88969859
ACCATGA48750.030.44272632
CAGTCAC49950.030.13143527
GCAATCT45650.029.74676339
AGCAATC47650.028.35131338
GTCACCA53100.028.01422129
TGCTTGA51750.027.93287560
TCTCGTA49750.027.64753243
Produced by FastQC (version 0.11.5)