FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4450305
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTAT79630.17893155637647307TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC13350.046.9284747
TATGCCG13100.046.75430748
CGTATGC13950.045.16093446
TCGTATG15850.039.30568745
ATGCCGT15900.038.74096349
CGTATCT17550.035.29847339
CTCGTAT17850.034.90168444
ATCCGTA19650.034.55404736
AGTCACG20250.034.39633628
GTCACGA23750.029.4747829
TCCGTAT22600.029.11481737
CCGTATC22000.028.95399338
CAGTCAC24350.028.74849727
GTATCTC21850.028.03150240
TGCCGTC22750.026.61457850
CCAGTCA27400.026.57092726
CTGCTTG20600.026.33474359
ACGTCTG29650.025.260915
CGTCTGA30500.024.7864116
ACTCCAG30650.024.78124823
Produced by FastQC (version 0.11.5)