FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5575695
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGTAT75340.13512216862651202TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAC24400.040.45263332
CGTATGC12600.039.44338246
GTAACCG25550.038.90271434
TATGCCG13400.037.87143348
GTATGCC13900.037.26518247
CACGTAA29050.034.3398131
TAACCGA29750.033.76349635
CGTAACC29500.033.45642533
AGTCACG31350.032.1551328
TCACGTA32350.030.94475730
TCGTATG15600.030.06329545
GTCACGT34250.029.33030929
CAGTCAC36450.027.5600327
ATGCCGT16850.027.20946149
ACCGAAT37350.026.42499237
CTCGTAT21100.024.88084844
CCAGTCA41750.024.64891626
ACGTCTG46150.022.37169515
ACTCCAG46850.022.33965123
CGTCTGA47850.021.72316616
Produced by FastQC (version 0.11.5)