FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4361468
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCTCGTAT82750.18972969651502658TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13650.045.63971746
GTATGCC13850.044.98066347
TATGCCG14300.044.05468448
TCGTATG14750.042.4733645
CGTTACA14800.040.91192636
GTCGTTA16550.039.3351234
TCGTTAC16850.038.21936435
ATGCCGT17350.036.10847549
GTTACAT16900.035.20730237
AGTCACG21000.030.83456828
CTCGTAT20550.030.82636644
TTACATC19700.029.66988238
TGCCGTC21950.028.8602250
CACGTCG23000.028.30613131
GTCACGT23300.027.94103429
TCACGTC23550.027.6444230
ACGTCGT23650.027.52690132
CTGCTTG21950.027.42549559
TACATCT21550.027.28522539
GCCGTCT23300.027.03785151
Produced by FastQC (version 0.11.5)