FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065712
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTAT67700.22082961478442856TruSeq Adapter, Index 4 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC9700.052.31862346
GTATGCC10650.047.65170347
TATGCCG10850.046.77256848
TCGTATG11800.042.41447445
ATGCCGT12600.040.55414649
ACAGACC12900.038.79963332
CAGACCT13800.036.52166433
TGCCGTC15400.034.08972550
CTCGTAT13850.033.10408444
GACCTTG15300.032.7123535
CACAGAC17000.030.47201531
GTCACAG17350.030.46152929
AGACCTT16850.030.11862834
GCCGTCT16750.030.08850751
CTGCTTG17400.028.5626959
CAGTCAC19200.027.89193227
CCTTGAT16600.027.40957837
AGTCACA19550.026.67644128
TGCTTGA18700.026.20272660
ACCTTGA18500.026.10805536
Produced by FastQC (version 0.11.5)