FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3505246
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTACATCTCGTAT78970.22529089256502965TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12650.051.7378146
TATGCCG12900.051.27776348
GTATGCC12800.050.85807847
TCGTATG14500.044.89540545
ATGCCGT15400.042.9534549
CCAAGGT17200.037.44197533
ACCAAGG17600.037.18920532
GGTACAT16900.036.44981437
CTCGTAT17600.035.59568444
CAAGGTA18200.035.38472434
AAGGTAC18550.035.09444835
TGCCGTC18800.034.99910750
AGGTACA18400.034.42945536
GTACATC17650.034.30507338
GCCGTCT19700.033.22250451
CTGCTTG19750.032.43001659
TACATCT19350.031.1103239
AGTCACC20900.030.98228328
CACCAAG22150.030.0243831
GTCACCA23200.028.66469829
Produced by FastQC (version 0.11.5)