FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8394761
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGTAT98950.11787113415140706TruSeq Adapter, Index 16 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA19700.045.12533
GCGTTAG20100.043.87874234
CGTATGC20000.041.9994846
GTATGCC20950.039.5937847
TATGCCG21050.039.5705448
CGTTAGA25300.035.1368635
CACGCGT26050.034.2630631
TCACGCG25750.034.25444830
TCGTATG24900.033.7345245
GTCACGC26550.033.222329
ATGCCGT26050.031.30367749
ACGCGTT31300.029.18650632
AGTCACG31550.028.7340428
CTCGTAT32350.025.64110644
TGCCGTC35600.021.82479150
CAGTCAC42700.021.2315227
ACGTCTG46200.020.60531415
CTGCTTG35400.020.36746459
GCCGTCT39050.020.25510651
GTTAGAA47200.019.35299736
Produced by FastQC (version 0.11.5)