FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4070572
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTAAGATCTCGTAT68820.16906714830249903TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12200.046.4737448
CGTATGC12350.046.1932446
GTATGCC12450.045.8222147
TCGTATG13700.041.1304145
ATGCCGT14150.040.5639149
CTAAGAT12950.040.26931437
CGTCCTA14250.039.78853233
TCCTAAG14350.039.02346435
CCTAAGA13750.038.43545536
GTCCTAA16200.034.7831334
CTCGTAT16800.031.87424544
TGCCGTC18450.031.48938450
ACGTCCT18350.030.90034732
CACGTCC18250.030.87825631
TAAGATC17200.029.30127138
GCCGTCT20050.028.4528451
CTGCTTG19600.028.39323259
AGTCACG20400.027.96708528
TGCTTGA23150.024.49276460
TCTCGTA21800.024.24254443
Produced by FastQC (version 0.11.5)