FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3771465
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAGATCTCGTAT57750.1531235209659907TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC9350.048.28788446
TATGCCG9500.047.1551548
GTATGCC11050.041.17571647
TCGTATG12250.036.28504645
ACCTCAG13250.036.19089532
ATGCCGT12600.035.5534949
CCTCAGA15700.030.7629533
TGCCGTC16250.028.6444950
CTCGTAT15500.028.22528844
GCCGTCT17300.026.29904751
AGTCACC18750.025.94859928
CACCTCA19600.024.46610631
CAGTCAC20550.023.50602327
CTGCTTG17400.023.33352359
TCTCGTA19850.022.21621543
ACGTCTG22850.022.20906815
GTCACCT22850.021.9050629
GATCTCG20050.021.12180541
CCAGTCA23300.021.03300526
CGTCTGA24750.020.64553816
Produced by FastQC (version 0.11.5)