FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4127756
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTAT73280.1775298733742983TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC13450.047.62068647
TATGCCG13800.046.66540548
CGTATGC13900.046.0790146
TCGTATG16050.039.9064345
ATGCCGT16650.039.09804549
GTCACGC16650.038.2595429
CACGCAA18850.034.16601631
CTCGTAT18200.033.8460644
TCACGCA19250.033.2738430
AGTCACG19600.032.6796728
TGCCGTC21200.030.87180550
ACGCAAT21150.030.45019332
CTGCTTG21750.028.16084159
GCCGTCT23900.026.9448851
CAGTCAC25600.025.97774727
TCTCGTA23350.025.33183743
TGCTTGA24900.024.59832860
CCGTCTT26450.024.3471752
CGCAATT27250.023.50423833
CCAGTCA29250.022.97540926
Produced by FastQC (version 0.11.5)