FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307818
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTACGCAATCTCGTAT32471.0548440961867076TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3250.064.61343445
TATGCCG3600.062.22034548
GTATGCC3600.062.22034547
CGTATGC3550.062.110846
CTACGCA4550.060.767435
ATGCCGT3700.060.5387249
TACGCAA4700.058.82801436
ACGCAAT4650.058.7079137
CGCAATC4350.057.92928738
TCTACGC4950.055.85690334
CTCTACG5000.055.29833233
CTCGTAT3950.054.04900444
ACTCTAC5300.052.82859432
CACTCTA5500.052.81659331
TGCCGTC4200.051.66510450
GCAATCT4350.051.492739
CAGTCAC5550.051.0795427
CCGTCTT4250.051.05728552
GCCGTCT4050.050.98611551
ATCTCGT4200.050.83179542
Produced by FastQC (version 0.11.5)