FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8354851
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAGTACATCTCGTAT176460.2112066391130135TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC26800.050.80144547
TATGCCG27050.050.46011448
CGTATGC27900.048.6730846
TCGTATG32200.042.7167345
ATGCCGT32000.042.43582549
AGTCACG41300.034.32314728
TGCCGTC39850.034.07644350
CTCGTAT41700.033.4047644
AGAGTAC42200.033.34071435
GTACATC41600.031.63355638
CTGCTTG44450.030.70910359
GCCGTCT44450.030.70744751
GAGAGTA45600.030.5477734
GAGTACA46900.029.25327736
GTCACGA49600.028.93238829
TCACGAG49050.028.8286630
AGTACAT47100.028.16304237
TACATCT46550.028.11935839
TGCTTGA50100.027.31576260
TCTCGTA50650.027.22563743
Produced by FastQC (version 0.11.5)