FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4614625
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTACCAATCTCGTAT82120.17795595525096838TruSeq Adapter, Index 15 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13250.045.96053748
GTATGCC13300.045.5255947
CGTATGC13550.044.94393546
TCGTATG14850.041.24513645
ATGCCGT16300.037.36055849
ACCTGTA17450.035.50240732
CCTGTAC18500.033.4855933
TGTACCA20850.030.5507435
GTACCAA20500.030.04797636
CTGTACC21150.029.95191834
TGCCGTC21800.028.7374550
CTCGTAT20650.028.13514544
CACCTGT22550.027.47303631
CTGCTTG22300.027.30957259
AGTCACC23300.027.1892928
TACCAAT23350.025.63100637
CAGTCAC25500.024.98107527
GCCGTCT25150.024.4921251
TGCTTGA26850.023.07275860
TCTCGTA25850.022.34006943
Produced by FastQC (version 0.11.5)