FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3690470
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGATTCATCTCGTAT60150.16298737017236287TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11950.048.9109346
TATGCCG11950.048.61739348
GTATGCC12050.048.21458447
ATGCCGT13650.043.0752849
TCGTATG13450.041.1142345
CTCGTAT16600.031.62572944
TGCCGTC18100.031.32477450
CACTCGG20650.029.15380131
CTGCTTG19300.028.29022859
GCCGTCT20950.027.06340851
GTCACTC23000.026.02321429
CTCGGAT23900.025.77307733
CAGTCAC24500.025.00209427
TCTCGTA20400.024.87683943
TCACTCG25350.024.43925730
TGCTTGA22150.024.01811860
AGTCACT25450.023.65556528
ACTCCAG26750.023.55369423
ACGTCTG27200.023.41822615
CCGTCTT24550.023.23740452
Produced by FastQC (version 0.11.5)