FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3518979
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGCATCTCGTAT72900.20716236158272047TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC11700.050.255547
TATGCCG11850.048.7319148
CGTATGC12500.045.6391846
TCGTATG12300.044.95853845
ATGCCGT13100.043.81475449
CTCGTAT16300.032.8521744
TGCCGTC17150.032.65154350
GTCACCG19700.031.44791829
AGTCACC20400.030.36925528
GCCGTCT18000.030.13748751
CAGTCAC22650.028.28004527
CTGCTTG18200.027.69259659
ACCGAAT23050.027.33255832
TCACCGA24750.025.59695230
CCAGTCA25250.025.5073926
TGCTTGA20850.025.01224960
TCTCGTA21200.024.76370243
ATTGCAT22950.024.55223537
CACCGAA25900.024.46040731
CCGTCTT22750.024.46040252
Produced by FastQC (version 0.11.5)