FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795401
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGAAGATCTCGTAT61280.2192172071198372TruSeq Adapter, Index 9 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10100.053.01732348
GTATGCC10150.052.41227747
CGTATGC10350.051.39948346
ATGCCGT11400.046.9714949
TCGTATG12300.043.5353345
TGCCGTC14500.036.9293150
CTCGTAT14200.036.72429344
GCCGTCT15700.034.99836351
AGTCACG16800.032.7097328
ACGAACG17800.031.0687432
CTGCTTG17350.030.46021759
TCTCGTA17200.029.91192843
CAGTCAC18750.029.86847527
CACGAAC18550.029.81312831
GATCTCG17300.028.929841
CGAACGA19550.028.10660633
GTCACGA20000.027.82618929
TGCTTGA18800.027.7385660
CCGTCTT21150.026.47630352
CCAGTCA21350.026.23203526
Produced by FastQC (version 0.11.5)