FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631961
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTATCATCTCGTAT71230.19611994732322294TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11200.051.56075746
TATGCCG11400.050.65617848
GTATGCC11900.049.11598647
TCGTATG13300.043.6827345
ATGCCGT13400.042.31200449
CCCAGTA15150.038.8132433
GTCACCC15300.038.66201829
CCAGTAT16700.035.629534
CACCCAG16750.034.89914731
ACCCAGT17650.033.51622832
TGCCGTC18250.032.21808650
GTATCAT18400.031.957237
CTCGTAT17750.031.35105344
GCCGTCT19150.029.79011751
AGTCACC19950.029.29967528
CAGTATC20350.029.2389535
CTGCTTG18700.029.19727559
ACGTCTG24200.027.91266315
CAGTCAC21700.027.74367127
TCTCGTA20250.027.4805543
Produced by FastQC (version 0.11.5)