FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4990366
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCTCCATCTCGTAT89440.1792253313684808TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC16600.049.96879247
CGTATGC16950.049.34995746
TATGCCG17100.049.3259248
TCGTATG18850.042.89031645
ATGCCGT19700.041.927649
TGCCGTC25200.032.6378550
CTCGTAT24750.031.67607744
GCCGTCT27100.030.09129351
CTGCTTG27600.029.29430859
GTCACTC30200.029.08993729
AGTCACT31700.028.15508828
TGCTTGA29100.028.14512860
CAGTCAC31900.027.75940527
CCGTCTT32600.025.55133452
CACTCAT35150.025.3924131
CCAGTCA36000.024.79311426
TCTCGTA31150.024.49384743
ACGTCTG38000.024.0387115
ACTCCAG39250.023.54277623
CGTCTGA39500.023.30352416
Produced by FastQC (version 0.11.5)