FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3096.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3096.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2861934
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTATCATCTCGTAT62230.2174403742364429TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9450.051.11047448
CGTATGC9700.050.51483546
GTATGCC10000.048.29939747
GCCTATC10950.044.4286735
ATGCCGT11100.042.8823549
CAGCCTA11400.042.67490433
TCGTATG11100.042.25172845
ACAGCCT11900.041.17595732
AGCCTAT12150.040.32871234
CTCGTAT12650.036.52128244
CACAGCC14000.034.9995631
TGCCGTC14600.032.3626150
GCCGTCT15600.030.96115551
TCACAGC16400.030.30502930
CTGCTTG15550.030.16039559
CCTATCA16300.029.63153336
GTCACAG16500.029.48499729
TCTCGTA15050.028.3717443
CCAGTCA18050.027.9240426
CTATCAT16050.027.47629237
Produced by FastQC (version 0.11.5)