FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4134668
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTAT100160.24224435916015505TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC15800.050.72587247
CGTATGC15800.050.28285246
TATGCCG16000.050.09119848
ATGCCGT18850.042.51772749
GACCTAT17950.042.31186737
TCGTATG18800.042.07283445
CTCGTAT20000.039.02348344
CCTATCT19750.037.7449139
TGCCGTC22450.036.01152850
ACCTATC22000.034.0437238
CTGCTTG23050.033.86067259
GTCACAG24300.033.85010529
CACAGTG24650.033.3702831
GCCGTCT24400.032.7032551
AGTGACC24850.032.53512634
GTGACCT25400.031.41724235
CTATCTC25450.029.5662740
TCTCGTA26000.029.34501343
AGTCACA28350.029.13819528
CAGTCAC29200.028.28999327
Produced by FastQC (version 0.11.5)