FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3094.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3094.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6267742
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCCAGATCTCGTAT121220.19340298308386017TruSeq Adapter, Index 7 (97% over 35bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT68340.10903448163628944No Hit
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT67460.10763046724003636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC19600.046.78429847
CGTATGC20100.046.14288346
TATGCCG19900.045.90276348
TCGTATG22600.040.88371745
ATGCCGT23350.039.4203349
TGCCGTC28650.032.37223450
GTCACTA29300.031.89475829
CTACTCC29450.031.84988433
ACTACTC30050.030.74978332
CTCGTAT29050.030.72196244
TACTCCA31200.029.726934
GCCGTCT32250.028.4330251
CACTACT33200.028.2539631
CTGCTTG31850.028.2415859
TCACTAC36450.025.73433330
CAGTCAC37200.025.1222127
GATCTCG34950.024.63444941
AGTCACT38600.024.5729728
TGCTTGA38200.024.37157260
TCTCGTA36600.023.81075343
Produced by FastQC (version 0.11.5)