FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585887
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGAGCTATCTCGTAT67660.1886841386803321TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11450.049.823646
TATGCCG11800.048.9389848
GTATGCC11950.048.6175647
TCGTATG13350.042.20827545
ATGCCGT13850.041.69530549
TAGGAGC14550.040.8920534
GGAGCTA14550.040.4109736
CTAGGAG14800.040.20130533
CACTAGG14900.039.22901531
ACTAGGA16050.037.29050432
GCTATCT14400.037.1862139
GAGCTAT15300.037.05753737
GTCACTA17700.033.61729
TGCCGTC18200.031.72967150
AGCTATC17400.031.37822538
CTCGTAT17100.031.31470344
CTGCTTG18150.031.23902359
GCCGTCT19500.029.61435951
AGGAGCT20050.029.50022535
TCACTAG21300.028.75687830