FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5154637
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTTCAATCTCGTAT95720.1856968783640827TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17250.047.6803546
TATGCCG17500.046.7973948
GTATGCC17900.045.7534147
TCGTATG19000.043.10452745
ATGCCGT21600.037.59049249
CTCGTAT22950.033.70313344
ACCAGGT25550.033.42635732
CACCAGG26850.031.54814731
TGCCGTC26250.031.06493250
AGTCACC27650.030.12899228
CCAGGTT29250.028.9556533
CTGCTTG29000.028.36351259
GCCGTCT29450.028.0459751
TCTCGTA28050.027.82510643
CAGGTTC31100.027.3457434
CAGTCAC30450.027.1288727
TGCTTGA31900.026.44335260
AGGTTCA32150.026.1260735
GTCACCA33250.025.26517929
TCACCAG35200.024.46218130
Produced by FastQC (version 0.11.5)