FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4548959
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTAT66880.14702264847847604TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC12050.045.89002647
CGTATGC12250.044.283746
GACCGTA15550.041.63889336
TATGCCG12950.041.6192748
AGACCGT18250.035.67039535
TCGTATG14900.035.23329545
AAGACCG20050.033.166334
ATGCCGT15950.033.13292349
CGTATCT17650.032.52012339
CCGTATC20900.030.142538
ACCGTAT21950.029.33936337
CTCGTAT18250.028.95758444
CAAGACC24650.027.2610233
TGCCGTC19600.026.06995650
CAGTCAC26450.025.93768327
AGTCACA26000.025.71347628
GTATCTC21650.025.54184340
ACGTCTG29800.024.19326215
GCCGTCT20200.023.38976751
CTGCTTG19550.022.91556559
Produced by FastQC (version 0.11.5)