FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4561303
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTCTATCTCGTAT120730.26468313988349385TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA18000.054.83358436
TATGCCG17000.052.2914948
CGTATGC17300.051.7898746
GTATGCC17750.050.47688747
GGTCTAT19300.049.50837
TCGTATG17300.047.94609545
TACGGTC21500.047.20951534
ACGGTCT22700.044.55967735
CTACGGT22750.044.30789633
CACTACG23200.043.75451731
ATGCCGT20250.042.6892149
GTCTATC21900.042.66908638
ACTACGG24150.041.74343532
GTCACTA25500.039.80584729
CTCGTAT23150.036.73721744
TCACTAC28150.036.30726230
TGCCGTC24450.034.7835350
ACGTCTG31200.033.5399815
CAGTCAC29650.033.40875627
CTATCTC27250.031.8516140
Produced by FastQC (version 0.11.5)