FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753535
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAGGTATCTCGTAT47400.12628095914917536TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9050.043.69950548
CGTATGC9050.042.92663246
GTATGCC9550.041.4121247
AGGTATC13150.036.7282338
TCGTATG10750.033.5336845
CCAGGTA15850.032.90058536
CAGGTAT15000.032.8983837
ATGCCGT11300.032.83024249
GGTATCT12600.032.49839839
ACGTCTG21450.025.61645115
CAGTCAC20750.025.1353127
TGCCGTC14700.024.76068550
CTCGTAT15050.024.41772744
AGTCACT22300.024.1721128
GTATCTC16150.024.0545440
CCAGTCA22400.023.90984726
CGTCTGA23550.023.1835716
GAACTCC24200.022.99902321
GCACACG24250.022.6592811
CACACGT24700.022.38815712
Produced by FastQC (version 0.11.5)