FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6487078
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGTAT102530.15805267024691239TruSeq Adapter, Index 16 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17550.050.2544548
CGTATGC18100.048.14766746
GTATGCC18850.046.60331747
TCGTATG21300.040.58558745
ATGCCGT21800.039.49387449
CACTCGT32750.030.88762531
CTCGTAT28100.030.6396144
TGCCGTC27950.030.17771550
CAGTCAC37400.028.16995627
CCAGTCA38750.027.0087326
AGTCACT38550.026.87521228
ACTCGTC38800.026.43203232
TCACTCG40250.025.82687430
GTCACTC39700.025.74385629
GCCGTCT31850.025.71328551
ACGTCTG42100.025.02316515
TCGTCTG41400.024.60162734
TCTCGTA34900.023.96773343
ACTCCAG46650.023.03534723
CTCGTCT45250.022.89519533
Produced by FastQC (version 0.11.5)