Basic Statistics
Measure | Value |
---|---|
Filename | HMJVKBGX7_n01_QG3086.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 4628703 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTAT | 6313 | 0.13638809835066107 | TruSeq Adapter, Index 4 (97% over 38bp) |
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT | 4878 | 0.10538589319729523 | No Hit |
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT | 4720 | 0.10197240998180268 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 1465 | 0.0 | 49.93012 | 48 |
CGTATGC | 1420 | 0.0 | 49.787117 | 46 |
GTATGCC | 1485 | 0.0 | 49.02198 | 47 |
TACCGAC | 2235 | 0.0 | 47.917908 | 35 |
CTTACCG | 2510 | 0.0 | 43.644005 | 33 |
ATGCCGT | 1615 | 0.0 | 42.475403 | 49 |
TTACCGA | 2615 | 0.0 | 41.088535 | 34 |
CCGACAT | 2780 | 0.0 | 37.26498 | 37 |
ACTTACC | 2955 | 0.0 | 37.073956 | 32 |
TCGTATG | 1605 | 0.0 | 37.070454 | 45 |
ACCGACA | 2920 | 0.0 | 35.95774 | 36 |
CACTTAC | 3275 | 0.0 | 33.879326 | 31 |
CAGTCAC | 3390 | 0.0 | 32.73144 | 27 |
TCACTTA | 3495 | 0.0 | 31.746721 | 30 |
GCGTATG | 590 | 0.0 | 31.43966 | 45 |
TGCCGTC | 2060 | 0.0 | 31.261124 | 50 |
AGTCACT | 3600 | 0.0 | 31.01523 | 28 |
ACGTCTG | 3710 | 0.0 | 30.47071 | 15 |
CGACATC | 2575 | 0.0 | 30.445616 | 38 |
CCAGTCA | 3715 | 0.0 | 29.869606 | 26 |