FastQCFastQC Report
Thu 12 Sep 2019
HMJVKBGX7_n01_QG3086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJVKBGX7_n01_QG3086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4628703
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTAT63130.13638809835066107TruSeq Adapter, Index 4 (97% over 38bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT48780.10538589319729523No Hit
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT47200.10197240998180268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14650.049.9301248
CGTATGC14200.049.78711746
GTATGCC14850.049.0219847
TACCGAC22350.047.91790835
CTTACCG25100.043.64400533
ATGCCGT16150.042.47540349
TTACCGA26150.041.08853534
CCGACAT27800.037.2649837
ACTTACC29550.037.07395632
TCGTATG16050.037.07045445
ACCGACA29200.035.9577436
CACTTAC32750.033.87932631
CAGTCAC33900.032.7314427
TCACTTA34950.031.74672130
GCGTATG5900.031.4396645
TGCCGTC20600.031.26112450
AGTCACT36000.031.0152328
ACGTCTG37100.030.4707115
CGACATC25750.030.44561638
CCAGTCA37150.029.86960626