FastQCFastQC Report
Wed 12 Jun 2019
HMJJGBGXB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJJGBGXB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535660835
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31924490.5959832773661714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT14815550.080.228721
AGCAGTG14899100.079.553322
GCAGTGG18185850.065.202183
CTCGATG2365100.064.31478
GCTCGAT2387200.063.5656747
GGCTCGA777900.061.9567346
CAGTGGT19219650.061.6422774
CGCTCGA538400.057.274876
CGGCTCG175900.056.693115
AGTGGTA21966150.054.148965
GTGGTAT25483850.046.7352376
GTATCAA28037400.042.3381089
GTCTATG3650500.042.1512767
GGGCTCG397850.041.8810425
CTATGCC3677300.041.5506979
TCTATGC3893300.039.8173758
AGGCTCG279300.035.8544965
CCGCTCG181550.035.5180475
CGTCTAT763450.034.642926
TGGCTCG362800.033.4202085
Produced by FastQC (version 0.11.5)