FastQCFastQC Report
Wed 12 Jun 2019
HMJJGBGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJJGBGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535660835
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA1844400.018.3515858
GGGACCT1397700.018.2515091
GCGCGAT1331850.017.968571
ACTGAAC1642900.017.938431
CGGACTG1494350.017.733881
CTGCGGA1232650.017.6260341
CACCGAA1209050.017.5047428
GATGCTA1687150.017.4866221
GGATTAC1675800.017.3913251
CGTGAGC1239200.017.3701271
ATAACGC1340500.017.2390941
ATGCGAT1401600.017.2287481
ATAGACC1382300.017.1752321
GAATGAA1517350.017.173641
TGACGGC1234450.017.1629831
GACTAAC1278500.017.1376951
AGGCGAC639950.017.119028
TGGGCGT1264050.017.1009391
ACTGATG1248350.017.0854661
CTCGAAA1370700.017.054681
Produced by FastQC (version 0.11.5)