FastQCFastQC Report
Fri 15 Dec 2023
HMJJFAFX5_n01_TopDonor1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJJFAFX5_n01_TopDonor1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45706697
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCACCACCCTGACCT1573960.3443609149880159No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC1330170.29102299822715255No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCACCACCCTGA1310210.28665602329566714No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCACCACCCTGAC1214940.26581225066427355No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT1104610.24167355606553673No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCACCACCCTGACC1102450.24120097761603732No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC1090590.23860617187017474No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT943560.20643801935633196No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAGTGCCGAAACA474030.10371127889639456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)