FastQCFastQC Report
Fri 15 Dec 2023
HMJJFAFX5_n01_BotDonor1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJJFAFX5_n01_BotDonor1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34686673
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCACCACCCTGACCT8260032.3813266841706033No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCACCACCCTGAC7142722.0592116170957073No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCACCACCCTGACC5890081.6980815657932948No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCACCACCCTGA5822421.678575515155345No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGATGCACTTTATTT891140.2569113503621405No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGATGCACTTTAT774570.2233047833673757No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCTGTCAAGTATTCC657290.18949352680783194No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC654230.1886113436131508No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGATGCACTTTA633430.18261480425061233No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGATGCACTTTATT632210.18226308415338652No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGTAGCCGGAGTGGT615890.1775581071150871No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC570370.164434911356301No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCTGTCAAGTATT562920.16228711240193028No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAGTGCCGAAACA559080.16118005898115395No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGTAGCCGGAGTG531710.15328942040650598No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAAAGTGCCGAAA482960.13923503127555645No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT474930.13692002112742263No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT470440.13562557585156698No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCTGTCAAGTAT465250.13412932396254895No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCTGTCAAGTATTC455680.13137033926545794No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGTAGCCGGAGTGG433770.12505379227347632No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGTAGCCGGAGT430960.12424368286921032No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAAGTGCCGAA398810.11497499342182514No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAAAGTGCCGAAAC397100.11448200869538569No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)