FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35164198
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11143243.1689162937826705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA280750.021.4773352
TCGGAAG289850.020.8733253
GATCGGA286150.020.629141
CGGAAGA296100.020.6120244
TCGTGTA153800.019.7397514-15
GAGCGTC344850.017.037899
AGAGCGT369850.016.0004758
TAGGGAA162000.015.38434218-19
AAGAGCG400200.015.1776187
GTAGGGA183050.015.09483718-19
AGCGTCG206450.015.01276810-11
AGGGAAA242700.012.41292420-21
CGTGTAT30500.012.22832514-15
GTGTAGG234050.011.8153716-17
GAAGAGC539000.011.6382046
ATTACGA28600.011.04525754-55
GGAAGAG591300.011.0092625
TGTAGGG232000.010.88549816-17
TATTACG21500.010.82624454-55
CGTGTAG266700.09.63765514-15
Produced by FastQC (version 0.11.5)