FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41089568
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT97950.013.8440331
AAAGGGG556750.011.82343380-81
CGCGATT13800.011.3777681
TAGGGGG182500.011.10084350-51
AGGGGGG743050.010.31000882-83
GGGGGGA764050.010.2282812
TAAAAAA1387300.09.99474770-71
AAGGGGG549700.09.61207182-83
AAAAAGG800900.09.5029878-79
GGGGGGT783900.09.33542614-15
CGGGAAT72900.09.2679361
CGGGGTT150350.08.9558161
CGGGGGG507950.08.86517212-13
CCGGGAT119450.08.8029511
AAAAGGG594100.08.73378580-81
CGGGATT73050.08.6627061
GGGGGAG729250.08.5478493
GGGTAAA217700.08.31475822-23
GGCGAAT73550.08.0862941
GGGAAAA623950.08.04837622-23
Produced by FastQC (version 0.11.5)