FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35790827
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT85100.012.9723891
AAAGGGG438750.010.59209880-81
GGCGAAT59750.09.5566321
CGCGATT13700.09.3778811
AGGGGGG607100.09.36814482-83
CGCGTAT6152.2333083E-59.2846951
TAGGGGG148100.09.14085850-51
CCGGGAT105150.08.5981661
TAAAAAA1126850.08.32404570-71
AAGGGGG421500.08.24805980-81
CGAATCG19200.08.1659823
CGGGAAT64000.08.104161
CGGGGTT142750.08.1001241
AAAAAGG669550.08.08293378-79
CGCGGAT38900.07.95106461
GTCGGAT32800.07.8339621
CTCGTAT28100.07.7895621
CGGGATT68350.07.7276241
CCGGAAT60400.07.72059351
GCGTCGT248550.07.710393494-95
Produced by FastQC (version 0.11.5)