FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35630912
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG491950.012.85456180-81
CGGGGAT123600.011.433531
CGCGAAA22750.010.6667251
TAAAAAA1119950.010.43405170-71
AAAAAGG702250.010.31030678-79
AGGGGGG791900.09.83284982-83
CGGGATT86600.09.28562451
AAGGGGG522100.09.274182-83
GCGAAAG105650.09.0975451
TAGGGGG171900.09.04964650-51
CCGGGAT101100.08.848081
AAAAGGG542250.08.8374678-79
GCGGAAT60650.08.5514151
GACATAA179200.08.4806455
GCGGTAT37100.08.3364621
GGGGGGA726450.08.2909372
GGGTTAA111050.08.1899666-67
GTCGATT38150.07.9822951
TAACCGA76100.07.896954516-17
ACGATCG21200.07.8438333
Produced by FastQC (version 0.11.5)