FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34617801
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG452450.012.74870180-81
CGGGGAT121600.011.1127411
CGCGAAA22200.010.7165131
TAAAAAA1131650.010.65984470-71
AGGGGGG681900.010.4474482-83
TAGGGGG172950.010.28554650-51
AAAAAGG660250.010.25765878-79
GGGGGGA673000.09.6204042
GACATAA173600.09.1373335
CGCGTAT6353.2590335E-58.9917441
AAGGGGG475350.08.932301582-83
AAAAGGG504050.08.92776478-79
GGGAAAA463100.08.63839722-23
CGCGATT12151.2732926E-108.6155471
CGGGGGG474450.08.61008212-13
GGGGGAA469000.08.22557920-21
CGCGAGT33950.08.1287671
GGGGGAG639000.08.0972463
GGGGGGT802800.08.05349214-15
GTCGCAT54750.07.90849261
Produced by FastQC (version 0.11.5)