FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32485894
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG408250.010.61548480-81
TAGGGGG151350.010.04296450-51
TAAAAAA934250.09.76288170-71
AGGGGGG584450.09.26378282-83
CCGGGAT108650.08.9345561
AAAAAGG557250.08.914885578-79
CGCGAAT14000.08.8372651
CGGGGAT106550.08.5747281
AAGGGGG404100.08.42089482-83
GTCGATT36500.08.2133481
AAAAGGG415600.08.13078680-81
GCGGTAT35300.07.81854341
CGGGAAT65400.07.7125981
GGGGGGA562950.07.692972
CGGGCAT136200.07.44174961
CTCGTAT29750.07.3577231
CCGGAAT73950.07.2713131
CGGGATT60000.07.21709971
GTCGGAT35700.07.19777251
CTCGGTT64900.07.185451
Produced by FastQC (version 0.11.5)