FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35675590
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG176250.012.10067450-51
AAAGGGG478100.011.99524180-81
TAAAAAA1091400.010.18501470-71
AAAAAGG654450.010.15992878-79
AGGGGGG677500.010.15782282-83
CGCGATT12151.8189894E-129.399291
AAGGGGG481250.09.094233582-83
CCGGGAT109200.09.0200261
AAAAGGG490650.08.99742480-81
GGGGGGA650950.08.7927322
CGGGGAT113100.08.708991
GGGGGGT703150.08.38409114-15
GGGAAAA450900.08.3240822-23
CGGGGGG450750.08.08806612-13
CGGGCAT145150.07.7039041
GGGGGAA493500.07.61009120-21
GGGAAAT295850.07.5432991
AAAAAAG959500.07.494034376-77
TATAGGG90150.07.482193548-49
GAAATCG88800.07.2768653
Produced by FastQC (version 0.11.5)