FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34295375
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG409300.011.6151980-81
CGATTCA108800.011.2633734
CGCGAAT14450.010.8672711
ACGATCG55100.010.7787053
CAACGAT155650.010.761381
GACATAA149050.010.5468885
GCAAAAG293900.010.4756061
ACGATTC118000.010.1467443
TAGGGGG158750.09.87407850-51
TAAAAAA951200.09.816380570-71
AAAAGGG416850.09.76421378-79
ATAACGT88900.09.7230476
TAACCGA127650.09.60176416-17
AGGGGGG648950.09.54699182-83
CGATCGA47850.09.5294314
CGCGTAT7001.0351087E-69.51709651
AAGGGGG416350.09.3766480-81
AGACATA187600.09.3426744
AAAAAGG583700.09.26359578-79
GATAACG95050.09.1937775
Produced by FastQC (version 0.11.5)